CATECHOLASE (TYROSINASE): AN IMPROVED METHOD OF PREPARATION
نویسندگان
چکیده
منابع مشابه
CATECHOLASE (TYROSINASE) :* REVERSIBLE INACTIVATION AND REACTIVATIONt
Studies of the chemical properties of the enzyme, catecholase, have shown that this polyphenoloxidase consists of a copper-protein complex (l-3). Indications have also been obtained that the enzymic activity of catecholase is associated with the copper part of the molecule, since it has been found that the addition of certain reagents known to react with cupric ions will inhibit the enzymic act...
متن کاملAn improved method for preparation of feces for bomb calorimetry.
was standardized before no. 1 and between the two no. 10 samples. The time required when the Auto Dilutor was used was: 3 mm to assemble equipment, 2.5 mm to label flasks, 9 mn to dilute standard and samples, and 8 mm to take readings. The on-line system only took 8 mm to run the standards and samples, saving 14.5 mm for the 10 duplicated determinations. No flasks are needed when an on-line dil...
متن کاملPreparation of Rat Gingival Mitochondria with an Improved Isolation Method
In order to establish a method of obtaining rat gingival mitochondria (Mt), Mt fractions were prepared in various combinations of homogenizing time with collagenase concentration. Rat gingival tissues were excised, minced, treated with collagenase, homogenized, and subjected to differential centrifugation rates. Both the respiratory control ratio (RCR) and adenosine diphosphate/oxygen (ADP/O) r...
متن کاملAn improved method of diet preparation for toxicological feeding experiments.
Groups of 30 rats each were fed 1 of 4 diets to determine feeding preference for freeze-dried meal compared to dry meal, and to determine if 3% agar is a desirable component of freeze-dried meal. The diets were: dry meal, meal + distilled water(freeze-dried), meal + 3% agar in distilled water(freeze-dried), and meal + 3% agar in distilled water(gel). Diet preparation, and the method for introdu...
متن کاملAn improved method for the preparation of protective pseudoglobulin.
Experiments indicating the existence of a menstrual toxin responsible for some of the clinical features of preeclamptic and eclamptic toxemias of pregnancy, and of a protective factor capable of neutralizing this toxin in animal experiments and in human cases have been reported by Smith and Smith (l-3). The apparent success obtained in some of the clinical cases in which protective pseudoglobul...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1942
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)45033-8